To evaluate whether elevated circulating Hsp70 and Hsp90 co-exist with cancer cachexia in vivo, we analyzed serum Hsp70 and Hsp90α levels in a syngeneic (LLC)4 and a genetic (Apcmin/+)9 mouse cancer cachexia model, as well as in mice bearing the non-cachexia-inducing EL4 tumor (Supplementary Fig. 2C), all of which were in the C57BL/6 mouse background. This evidence concerns the gene HSP90AA1 and neoplasm.