Utilizing enzyme-linked immunosorbent assay (ELISA), we detected very low levels of Hsp70 and Hsp90α in conditioned media of the non-tumorigenic cells as well as EL4 lymphoma cells lacking the capacity to induce cachexia, whereas the content of Hsp70 and Hsp90α in conditioned media of the cachexia-inducing tumor cells were dramatically elevated by averaging ~10-fold (Fig. 1a). This evidence concerns the gene HSP90AA1 and Cachexia.