Quantitative analysis showed that Trak1 hyrt mutant had significantly reduced colocalization with the mitochondrial marker TOM20 than that of Trak1 WT or endogenous Trak1 (Fig. 3E), suggesting that the localization of Trak1 to mitochondria is partially impaired by hypertonia-linked Trak1 mutation. The gene discussed is TRAK1; the disease is Hypertonia.