We isolated pan-DCs (CD11c+ MHC-II+), MDSCs (CD11b+ Gr-1+) and the remaining CD11b+ CD11c− Gr-1− myeloid population from tumours and spleens of melanoma-bearing WT mice at day 12 post-tumour cell injection (Fig. 2a). The gene discussed is ITGAM; the disease is melanoma.