To determine the mitogenic effects of OGN on meningioma cell proliferation, we stably transfected OGN constructs into IOMM-Lee, a malignant human meningioma cell line with minimal levels of endogenous OGN, with confirmation of mRNA expression by qPCR (Fig. 2a) and protein expression by Western blotting and IHC (Fig. 2b-c). The gene discussed is OGN; the disease is meningioma.