Western blotting analysis indicated that the phosphorylation of mTOR and its downstream signals, eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), eukaryotic translation initiation factor 4B (eIF4B), and eukaryotic elongation factor 2 kinase (eEF2K), were all elevated in OGN-overexpressing meningioma lines (Fig. 5a). This evidence concerns the gene EIF4B and meningioma.