To determine whether the effects of OGN in IOMM cells are mediated by NF2, NF2 expression at the transcription and translational level was evaluated by qPCR and Western blotting following transfection of OGN. NF2 mRNA expression was attenuated in OGN-expressing meningioma cells (Fig. 4a), with corresponding subsequent decrease in NF2 protein level (Fig. 4b). Here, NF2 is linked to meningioma.