Along this line, it would be beneficial to evaluate the basal ratio of O-MAD2 to C-MAD2, or MAD1 to MAD2, or the ratio of checkpoint silencing proteins such as TRIP13 and p31comet to MAD2, in different cancer cell lines [45,166], as these ratios may help determine C-MAD2 and MCC levels in response to mitotic checkpoint signaling. The gene discussed is TRIP13; the disease is cancer.