Moreover, even though we found a direct inhibition of the TCF/Lef-mediated transcriptional activation in HCT116 but not in DLD1 and SW620 cell lines, in several models of human CRC the compound was able to decrease the expression of well known β-Catenin target genes (Fig. 5c and see Supplementary Fig. S5). This evidence concerns the gene HNF4A and colorectal carcinoma.