To further objectively compare the phenotypic profiles of tumour specific cells derived from tumour vs. periphery with and without anti-CTLA4 treatment across separate experiments, we averaged the median expression intensities from independent experiments (n = 5) for each of the non-lineage molecules assessed and calculated z-scores for each phenotypic marker and averaged them for each T-cell specificity as previously described23. Here, CTLA4 is linked to neoplasm.