To determine whether the phenotypic alterations of mLama4- and mAlg8-specific TILs also occurred in the periphery of anti-CTLA-4-treated tumour-bearing mice, we used data derived from peripheral CD8+ T cells to broadly analyse the resultant changes in the dominant neoantigen-specific CD8+ T-cell population from the two treatment groups using the aforementioned t-SNE-based dimensionality reduction approach. Here, CTLA4 is linked to neoplasm.