In this study, new puzzling questions have been revealed and remain unanswered, including; (i) what is the actual translational start(s) of AinS, considering previous annotations are inconsistent with transcripts arising at TSS2, (ii) what is the mechanistic role of IR1 in ainR, and (iii) is the negative feedback loop between LuxR and ainSR relevant to PS during symbiotic infection. The gene discussed is NISCH; the disease is infection.