Analysis of the gold particle distribution indicative of HSP90 protein (n = 30 sarcomeres) confirmed the preferential I-band binding (~50% of gold particles) in myopathy and also showed additional minor associations with the A-band (25%) and (only in filaminopathy muscles) the Z-disc (22%) (Additional file 1: Figure S3 c). The gene discussed is HSP90AA1; the disease is myopathy.