Ten biochemical parameters related to diabetes and obesity were measured by a multiplex kit: c-peptide, ghrelin, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1), glucagon, insulin, leptin, plasminogen activator inhibitor-1 (PAI-1), resistin and visfatin. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.