Moreover, targeted deletion of both p53 and Pten [67] using an adenovirus expressing Cre recombinase delivered directly into the bladder of mice with floxed alleles of p53 and Pten (p53fl/flPtenfl/fl), developed bladder tumors with 100% penetrance after 24 weeks, with metastasis to local lymph nodes and distant sites, including the spleen, liver and diaphragm. Here, TP53 is linked to urinary bladder neoplasm.