When CM was analyzed by gelatin and plasminogen zymography, reduced levels of 72/92 kDa (MMP2/MMP9) and 70/45kDa (tPA/uPA) zone clearing activity were detected from daurinol treated MDA-MB-231 and A549 cancer cells compared to the control in a concentration-dependent manner (Figure 4A). The gene discussed is MMP9; the disease is cancer.