BECN1 and cancer: Further, we co-treated the cancer cells with either DET or DETD-35 and measured cell viability, monitored cell morphology, and examined beclin-1 (an early stage marker of autophagy), LC3 (microtubule-associated protein 1 light chain 3, an autophagosomal marker) and p62 (a marker of autophagic flux at a later stage of autophagy) protein expressions in MDA-MB-231 cells.