To ascertain that CaSR activation in tumor cells is responsible for decreased osteoblasts OPG production, SaOS2 and HOB cells were exposed for 24 hours to conditioned media prepared from CaSR-WT-, CaSR-DN- and EV-transfected cells exposed to 5 mmol/L of Ca2+ for 24 hours. This evidence concerns the gene CASR and neoplasm.