Compared with PD98059, the inhibitory effects of LY294002 were more pronounced, suggesting that PI3K/Akt activation is more important for pancreatic cancer cell development, which was further supported by the FACS results that LY294002 caused more remarkable apoptosis than PD98059 to BxPC-3 and PANC-1 cells (Supplementary Figure 2C). Here, AKT1 is linked to pancreatic neoplasm.