To validate the phosphoproteomic findings and evaluate the state of 4E-BP1 phosphorylation on Thr 37/46, lysates prepared from HuH7 WT cells and HuH7 cells stably expressing core protein variants either isolated from tumor cT or non-tumor cirrhotic (cNT) areas, were analyzed by immunoblotting using an antibody that recognizes exclusively the dual phosphorylation on the residues Thr37/46. Here, EIF4EBP1 is linked to neoplasm.