In order to determine the importance of the GABARAPL1 conjugation to autophagosomes on its tumor suppressive function, we designed MCF-7 breast cancer cell lines overexpressing either Flag:GABARAPL1:6His (GABARAPL1) or Flag:GABARAPL1-G116A:6His mutant (clone 1 and clone 2 ; GABARAPL1 G116A c1 and c2) (Figure 1A). Here, GABARAPL1 is linked to breast carcinoma.