To investigate the role of GABARAPL1 conjugation to autophagosomes on its tumor suppressive function, we designed two in vitro models: one MCF-7 cell line stably overexpressing GABARAPL1 and two stably overexpressing the GABARAPL1 G116A mutant protein which is no longer able to be linked onto phospholipids [23]. Here, GABARAPL1 is linked to neoplasm.