GABARAPL1 and breast cancer: To do so, the luminal BT474 breast cancer cell lines was first subjected to transient transfection with the Ctrl, the Flag:GABARAPL1:6His (GABARAPL1) or the Flag:GABARAPL1-G116A:6His (G116A)-expressing vectors (Supplementary Figure S4A) and then analyzed using proliferation and migration assays (Supplementary Figure S4B and C).