For HCT116 and SW480 colon cancer cell lines, in which β-catenin or APC is mutated [14–16], ICC experiments (Figure 2E, 2F) revealed similar phenotypic changes showing that SMYD2 knockdown strikingly decreased the amount of nuclear β-catenin, implying that SMYD2-mediated β-catenin methylation is essential for β-catenin nuclear translocation regardless to the dysfunction in the β-catenin ubiquitination machinery or β-catenin activation by the β-catenin mutation. This evidence concerns the gene SMYD2 and malignant colon neoplasm.