To investigate the importance of β-catenin methylation in regulation of the Wnt/β-catenin/TCF pathway, we performed quantitative RT-PCR to examine mRNA levels of some pivotal downstream genes in this pathway, including cyclin D1 (CCND1) and cMYC, which are well known for their involvement in cell cycle progression and proliferation of cancer cells. The gene discussed is CCND1; the disease is cancer.