When considering the total and phosphorylated expression levels of proteins analysed in basal conditions (no insulin stimulation), only the levels of phosphorylated ERK1/2 were significantly higher in DM and T2DM myotubes as compared to CTR (S3 Fig; p-T202-ERK1: CTR vs DM1 p<0.0001, CTR vs DM2 p<0.0001, CTR vs T2DM p = 0.03; p-Y204-ERK2: CTR vs DM1 p<0.0001, CTR vs DM2 p<0.0001, CTR vs T2DM p = 0.02). Here, INS is linked to diabetes mellitus.