In CTR myotubes, activation of IRS1 was evident starting from 5 minutes and persisted till the end of the experiment (5 minutes: CTR vs DM1 p = 0.002, CTR vs DM2 p = 0.004; 15 minutes: CTR vs DM1 p = 0.01; CTR vs DM2 p = 0.02) (Fig 5A and 5B). This evidence concerns the gene IRS1 and myotonic dystrophy type 1.