It is known that DAG is metabolized to AG by action of the ghrelin O-acyltransferase (GOAT); not only does the importance of this enzyme lie in its ability to acetylate the unacetylated form of ghrelin, but it has been reported that knocking down the GOAT gene protects mice from obesity induced diet, improves insulin sensitivity, and reduces adiposity when fed HFD and high glucose diet [74]. Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.