In this study, the protein expression profile regulated by IL-17A was isolated and identified in the oxygen-glucose deprivation (OGD) model of microglial cells using two dimensional (2D) gel electrophoresis and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) technology, and the signaling pathway in which IL-17 functioned was validated in vitro, which provides new insights into therapy of cerebral ischemia. Here, IL17A is linked to Cerebral ischemia.