A technical validation of microarray platform by methylation-specific PCR was performed on 5 differentially methylated genes (FGFR2, HOXD10, RASAL3, CYP1B1 and ITIH5) involved in key biological pathways of cancer development (e. g. MAP/ERK, Homeobox signaling and FGF signaling pathways). The gene discussed is ITIH5; the disease is cancer.