In addition, we found a significant downregulation of miR-490-3p in COPD fibroblasts after stimulation with 2.5 ng/ml (p<0.01, Fig 3A) and a significant induction of miR-143-3p in COPD fibroblasts after stimulation with 7.5 ng/ml TGF-β1 (p<0.01, Fig 3B), which both were not observed in the replication of the control subjects. This evidence concerns the gene TGFB1 and chronic obstructive pulmonary disease.