To examine RRM2-c2orf48 expression in mRNA levels of NPC, we performed RRM2-c2orf48 quantitative RT-PCR (qRT-PCR) in NN01–09, immortalized NPECs and NPC cell lines, which include CNE1, CNE2, 6-10B, 5-8 F, HONE1, SUNE1 and HNE1 cell lines. The gene discussed is RRM2; the disease is nasopharyngeal carcinoma.