In previous studies targeting the TLR-4-dependent signaling cascade and tumor necrosis factor-α signaling, we showed that even during the onset and very early stage of NAFLD, e.g., steatosis without inflammation, disrupting the TLR-4 signaling cascades was associated with lower fat accumulation [18,19,36], suggesting that an activation of these cascades may already be critical to the development of steatosis. This evidence concerns the gene TNF and steatosis.