We investigated autophagy markers and found that IMM‐H007 administration increased the expression levels of Beclin‐1 and PI3K, as well as the ratio of LC3B‐II : I, but decreased the level of insoluble p62 in HFD‐fed hamsters, indicating that IMM‐H007 does contribute to autophagy in HFD‐induced NAFLD. The gene discussed is BECN1; the disease is metabolic dysfunction-associated steatotic liver disease.