The TZM-bl cells are of epithelial origin, and the acute T cell leukemia (ATCL) derived Jurkat T cells, like primary human CD4+ T cells, are suspension cells with expression of co-receptor CXCR4 and CCR5 on cell surface, which can be a perfect option for HIV-1 infection study. Here, CXCR4 is linked to HIV-1 infection.