Therefore, to examine whether exosomal DNA (exoDNA) could be utilized as a surrogate for tumor tissues or cells in the detection of tumor-specific genetic mutations just as it is with ctDNA, researchers have tested the exoDNA isolated from various cancer cell lines to detect the BRAF (V600E) mutation and anti-epidermal growth factor receptor (EGFR) mutation, using allele-specific polymerase chain reaction (AS-PCR). This evidence concerns the gene BRAF and neoplasm.