Mutation frequency in tumor tissues from rb1 TALEN-injected F0 tp53e7/e7 zebrafish varied from 66.6% (32/48) to 83.3% (40/48), while the frequency in cdkn2a/b TALEN-injected F0 tp53e7/e7 zebrafish varied from 81.3% (39/48) to 91.6% (44/48) (Figure 6A). This evidence concerns the gene CDKN2A and neoplasm.