After 48 h incubation with IFN-γ, PD-L1 expression was upregulated at different levels on two of the three cutaneous melanoma cell lines (MEL13.03, MEL93.05) and on two of the three CM cell lines tested (CRMM2 and CM2005.1), while PD-1 was only slightly increased on one cell line (CRMM2) (Figure 6). The gene discussed is PDCD1; the disease is cutaneous melanoma.