Notably, as shown in Figure 4F, we observed that the SD phenotype was less represented (32.0% vs 13.3%), and the AD phenotype was enhanced (17.0% vs 31.3%), after ectopic expression of Ngn3 in ZEB1/231 cells; this demonstrated that ZEB1 is linked to stem cell identity in breast cancer cells, thus at least partially accounting for the downregulation of Ngn3 expression. This evidence concerns the gene NEUROG3 and breast cancer.