In order to clarify the role of TF in regulating the proliferation and promoting apoptosis of HCC cells through the regulation of COX-2-Wnt/β-catenin signaling pathway, PCR was applied to determine the mRNA expression of GSK-3β, Akt, VEGF, COX-2 and β-catenin, and protein expression levels of GSK-3β, p-GSK-3β, Akt, p-Akt, VEGF, COX-2, β-catenin and p-β-catenin were determined by Western Blot. This evidence concerns the gene GSK3B and hepatocellular carcinoma.