ACTA1 and listeriosis: We used five pregnant C57BL/6 female mice at 16 days of embryonic gestation (E16) (n = 5), as a model of neonatal listeriosis and challenged them intravenously (i.v) or not (not infected; NI) with 3000 CFU/mL of wild-type GFP-LMWT (LMWT), GFP-LM∆LLO (LM∆LLO) or GFP-LM∆ActA (LM∆ActA) mutants that encode LLO and ActA virulence factors, respectively.