In this study, we analyzed the levels of peripheral plasma DKK1 and AFP and the percentage of TEMs with respect to the sensitivity, specificity and AUC of each biomarker alone and a combination of two or three biomarkers in HCC patients with hepatitis B virus (HBV) infection and patients with HBV-related liver cirrhosis (LC), patients with chronic hepatitis B infection (CHB), and healthy controls(NC). The gene discussed is DKK1; the disease is laryngotracheoesophageal cleft.