Annexin V-PE/7-AAD analysis also showed that aspirin and sorafenib-induced apoptosis was more effective for the ACSL4+GADD45B− cells than for the ACSL4−GADD45B+ cells (Supplementary Figures S3B and C), thus suggesting ACSL4 and GADD45B may play critical regulatory roles in mediating the synergistic therapeutic effect of aspirin and sorafenib in HCC cells. Here, ACSL4 is linked to hepatocellular carcinoma.