ANXA5 and hepatocellular carcinoma: Annexin V-PE/7-AAD analysis also showed that aspirin and sorafenib-induced apoptosis was more effective for the ACSL4+GADD45B− cells than for the ACSL4−GADD45B+ cells (Supplementary Figures S3B and C), thus suggesting ACSL4 and GADD45B may play critical regulatory roles in mediating the synergistic therapeutic effect of aspirin and sorafenib in HCC cells.