Therefore, the present study aimed to find out cancer-specific hot spot mutations in five major cancer-related genes KRAS exon1 &2, NRAS exon 1, PIK3CA exon 20, IDH2 exon 4, and EGFR exon 20 in North Indian GBC patients and their association with clinicopathological variables through a hospital-based study. Here, NRAS is linked to cancer.