In summary, our data, although limited, demonstrated that Capillarys 2FP HbA1c system could report accurate HbA1c results in thalassemia silent/trait, and HbA2 concentrations (≤ 2.2% for α-thalassaemia silent/trait and > 2.8% for β-thalassemia trait) and abnormal bands (HbH and/or HbBart’s for HbH disease, HbF for β-thalassemia) may provide valuable information for screening. The gene discussed is HBA2; the disease is thalassemia.