We then further validated the sensitivity of the assay to detect SNVs (called a variant in relation to reference genome hg19) and performed smMIP analysis on RNA, isolated from the IDH1R132H mutant oligodendroglioma line E47848 and the astrocytoma cell line E98, in which we previously identified a novel mutation in IDH1 (IDH1R314C)35. This evidence concerns the gene IDH1 and astrocytoma (excluding glioblastoma).