Conformation by ELISA study in a cohort revealed that seven (CDH5, Trappin-2, BMP-4, IGFBP-6, Siglec-5, TIMP-4 and Decorin) of the 11 plasma proteins were significantly elevated (1.1–2.9-fold) in T2DM patients with CLI compared to those without CLI. This evidence concerns the gene PI3 and type 2 diabetes mellitus.