In addition to BT474 cell-line and HeLa cell-line, clinical whole blood samples from three TNM stage IV colon cancer patients were also successfully stained in 35 minutes with mixed stains such as Hoechst, EpCAM-FITC and CD45-PECy7 by using this 3D-μDialysis chip after pre-screening out erythrocytes, and the result shows a decent 2.16 signal to noise ratio of EpCAM-FITC suggesting a promising tool for utilization in real clinical applications. The gene discussed is PTPRC; the disease is malignant colon neoplasm.