By contrast, of the 4 dysplasia samples, LD1 shared 42 mutations with the 4 ESCCs, including the well-known ESCC related genes TP53, NFE2L2, and RB1, while the LD2, HD3, and HD4 had nearly no overlapping mutation with all 4 ESCCs. The gene discussed is RB1; the disease is esophageal squamous cell carcinoma.