Together, these data indicated that there was aberrant regulation of Chk1-CDC25C-CDK1/CyclinB1 and P53-P21WAF1-CDK1/CyclinB1 signaling pathways transduction in ovarian cancer cells, and only the activity of CDK1 protein, not the expression of CDK1 protein was regulated by the CDC25C signaling and P53 signaling pathway. Here, TP53 is linked to ovarian cancer.