NUSAP1 and astrocytoma (excluding glioblastoma): The 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that the NUSAP1-transduced astrocytoma cells (SW 1088 and A172 cells) displayed a significant increase in viability, while the NUSAP1-silenced U-87 MG and A172 cells displayed contrasting results (Fig. 3c and Additional file 3: Figure S2B).