NUSAP1 and astrocytoma (excluding glioblastoma): We analyzed the expression of NUSAP1 in astrocytoma samples which were selected from low grade glioma (LGG) data of the TCGA databases to perform the survival analysis and found that astrocytoma patients with high NUSAP1 expression had poorer overall survival than patients with low NUSAP1 expression (Fig. 2a).