In addition, Muscogiuri et al., studied 168 elderly subjects (mean age of 82 years) and showed a significantly higher prevalence of AITD in subjects with vitamin D deficiency (25(OH)D level < 50 nmol/L) (p = 0.002) and a significant correlation between 25(OH)D and anti-TPO levels (r = −0.27; p = 0.03) [31]. This evidence concerns the gene TPO and vitamin D deficiency.