ACSL4 and Schnyder corneal dystrophy: To gain further insight into the functional differences between ACSL1 and ACSL4, and to elucidate the metabolic features corresponding to the ACSL/SCD network enzymes, we performed metabolomics analysis using ACSL1 and ACSL4 cells together with the corresponding DLD-1 cells overexpressing SCD (SCD cells) and the three enzymes at the same time (x3 cells)19.