Fifth, to confirm the relationship between autophagy and human glioblastoma cell death induced by SH, we treated U87 and SF767 cells with SH (0.5 mM) for 48 h, and the percentage of viability in the two cell lines was found to be enhanced in Beclin-1 or ATG5 knockdown cells (Figure 2G) as well as the cells pretreated with the autophagy inhibitor (CQ, 3-MA, wortmannin (WORT)). This evidence concerns the gene BECN1 and glioblastoma.