In the present study, PTEN expression was up- regulation when SALL4 was reduced by siRNA-SALL4, as a result, the inhibitory action of PTEN on PI3K/AKT signaling was weaken, thus receded the cycling D1 level which arrested the cell cycle at G1 phase, regulating glioma proliferation. This evidence concerns the gene SALL4 and central nervous system cancer.