In the present study, PTEN expression was up- regulation when SALL4 was reduced by siRNA-SALL4, as a result, the inhibitory action of PTEN on PI3K/AKT signaling was weaken, thus receded the cycling D1 level which arrested the cell cycle at G1 phase, regulating glioma proliferation. The gene discussed is SALL4; the disease is glioma.