EGFR and colorectal adenocarcinoma: We selected EGFR-positive cancer cell lines including A549 human lung carcinoma cell, DU145 human metastatic prostate cancer cell, HeLa human cervical carcinoma cell, HT29 human colorectal adenocarcinoma cell, SH-SY5Y human bone marrow neuroblastoma cell, and SK-OV(3) human ovarian carcinoma cell lines25–29.