To assess HR functionality, we evaluated the baseline levels of RAD51 protein expression and foci formation in the PTEN-mutated and wild-type endometrial cancer cells upon DNA damage, with 1 h treatment of 500 nM doxorubicin, by immunofluorescence, as an indication of the cells ability to repair DNA double strand break [51]. The gene discussed is PTEN; the disease is endometrial cancer.