In order to study the effects of Foxy-5 on tumor growth and metastatic spreading in vivo, immunodeficient NMRI nude mice were injected orthotopically with cells expressing low levels of WNT5A (DU145-Luc) or with cells expressing higher levels of WNT5A (PC3M-Luc2). The gene discussed is WNT5A; the disease is neoplasm.